Untargeted Lipidomics and Metabolomics
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Liquid chromatography (reversed phase) – Mass spectrometry based untargeted metabolomics
This platform enables detection of hydrophilic metabolites and largely covers secondary metabolites from plants and microorganisms. 700 - 3000 features are detected and around 120-695 metabolites annotated. Annotation (identification) is performed by matching the internal database (a growing database presently containing information of more than 8000 compounds) exact mass and retention time. This platform enables detection of polyamines, amino acids, amino acid derivatives, nucleotides, nucleosides and their derivatives, saccharides, coenzymes and precursors (e.g. nicotinic acid, riboflavin, biotin), dipeptides, glutathione, plant and microorganisms’ specific secondary metabolites (flavonoids, alkaloids, tannins, Aflatoxins etc.), neurotransmitters from different chemical groups, semi‐lipophilic compounds like bile acids, acyl-carnitines and saponins.
To increase the number of detected and annotated compounds the measurements are carried out in positive and negative ionization modes.
Untargeted metabolomics can be focused on certain groups of compounds, pathways, classes.
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