Annotation Using Fragmentation Spectrum
ANNOTATION OF COMPLEX LIPIDS USING FRAGMENTATION SPECTRUM (LC-MS/MS )
Usually 10-30 % of all detected lipids get annotated (identified) during untargeted lipids profiling analysis.
MetasysX offers untargeted lipidomic for holistic studies of lipidome profiling. The golden standard methodology is High-resolution mass spectrometry (HRMS) in combination with Reversed Phase Ultra Performance Liquid Chromatography (RP-UPLC).Lipid species are separated by their hydrophobic fatty acyl moieties; hydrocarbon chains attached to the lipid core structure (like glycerol in triglycerides or sphingosine in sphingolipids). The length and degree of saturation of these chains determine the hydrophobicity of the lipid. Data is acquired in the Data Dependent Acquisition (DDA) method. A precursor scan (full scan, MS1) is performed to determine the mass-to-charge ratio (m/z) and abundance of ions, from which the most intense precursors ("top N") are isolated for MS/MS (MS2) fragmentation. The core for high precision and wide-ranging annotation of the lipid classes is the detection of lipid-specific fragmentation patterns in LC-MS/MS data.
The comprehensive annotation of high-resolution tandem mass spectral untargeted lipidomic data is performed by MetasysX internally developed Software combined with our in-house LC-MS/MS database of complex lipids (see. Lipid classes). MetasysX in house database covers the information of accurate mass values (mass to charge, m/z), retention times and an associated MS/MS- lipid spectra library.
The data acquisition in LC-MS/MS is performed positive and negative ionization modes. Our When combined with annotation using the MetaSysX database, this approach enables the relative quantification of hundreds of lipid species.
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