Lipid Classes
Free Fatty acids
FA
Diacylglycerol
DAG
Triacylglycerol
TAG
Phosphatidylcholine
PC
Phosphatidylinositol
PI
Phosphatidylethanolamine
PE
Phosphtidylglycerol
PG
Lysophosphatidylcholine
LysoPC
Lysophosphatidylethanolamine
LysoPE
Lysophosphatidylglycerol
LysoPG
Ether-linked phosphatidylcholine
ePC
Ether-linked Phosphatidylethanolamine
ePE
Ether-linked Lysophosphatidylcholine
eLysoPC
Sphingomyelins
SM
Ceramides
Cer
Hexosylceramide
HexCer
Inositol phosphorylceramide
IPC
Cardiolipins
CL
Sterol Esters
SE
Acyl carnitines
AC
Monogalactosyldiacylglycerols
MGDG
Digalactosyldiacylglycerols
DGDG
Sulfoquinovosyl diacylglycerols
SQDG
Cholic acid, Sphingosine
In the first stage, the spectral (mass to charge ratio of detected ions) and chromatographic information is matched to the internal database, which contains m/z and RT of over 1500 lipids from different lipids classes.
In the second stage, an additional level of annotation from the fragmentation spectrum (LC-MS/MS) is applied. In this approach, information about diagnostic fragments and precursor ions is used to reliably annotate lipid species. Further information from two annotation approaches is combined to achieve increased coverage of lipids.
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