Targeted Metabolomics by Mass Spectrometry
On request, MetaSysX offers targeted metabolomics using either LC-MS or GC-MS, to quantify your compounds of interest.
To ensure the quality, we analyze pure standards alongside biological samples across a range of concentrations.
Standard curves are generated within the same analytical batches as the samples, allowing robust and reliable quantification.
Targeted analysis is available only for compounds that are accessible as pure reference standards. These can either be provided by the customer or purchased directly by metaSysX. We also offer the use of stable isotope-labeled standards. In such cases, the internal standard is spiked into each sample prior to extraction using one of our validated protocols. The labeled form must be available.
Each analysis is optimized so that the concentrations of both the standard and the compound in the biological sample are within the detector's linear response range.
Please note that this service is currently available for a limited number of compounds. Reach out to discuss availability and feasibility for your specific targets.
While we often know which metabolites are extractable and detectable under given conditions, successful detection still depends on whether the compound is present in the sample above the limit of detection (LOD) and the limit of quantitation (LOQ).
As part of the service, we also calculate absolute concentrations (e.g., in mg) relative to the sample amount (e.g., grams, milliliters, or cell count). Additionally, we can estimate extraction efficiency by analyzing pure compounds processed through our extraction workflow.
Although LC-MS- and GC-MS-based targeted approaches using standard curves are useful for absolute quantification, they have its limitations.
The analysis can be performed using GC-MS or LC-MS.
We perform the analysis by measuring compounds of interest in different concentrations together with biological samples. The standard curves are measured in the same batches as the samples in which a compound of interest should be quantified.
The analysis can be performed only for compounds that are available as pure standards. It can be either purchased by metaSysX or delivered by the customer.
We can also perform the targeted analysis using standards that are isotopically labeled with stables isotopes. The internal standard will then be spiked into the samples and the samples will be extracted using one of our extraction methods.
We optimize the analysis to have the standard in the range of the biological sample and within the linear range of the detector response for a compound.
Please, ask about this service as this is offered for a limited number of compounds.
We know often which metabolites are extractable and detectable, but the detection depends on the presence of the compound above the limit of detection and limit of quantitation.
We also perform the calculation to obtain information of absolute amounts (in mg) per amount of a sample (in g, ml or number)
We can estimate extraction efficiency of a compound by performing extraction of a pure compound.
Using LC-MS and GC-MS- based approaches and standard curves of pure compound for absolute quantitation (targeted metabolomics) have limitations.
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